genea P. falciparum M76611b coordinates T. parva Z23263 coordinates Raabe, et al.29 cDNAsc size RNA/DNA (P. falciparum)d subunit/ordere
SSUA 2023-1916 3666-3561 mtR_6043/27 125/108 SSU/4
SSUB 505-390 4182-4074 none complete 116/116 SSU/6
SSUD 5446-5379 3800-3733 mtR_111/36 65/68 SSU/10
SSUE 1638-1680* 2095-2057 mtR_215/7 40/42 SSU/11
SSUF 5507-5447 1840-1900 mtR_11b/1105 74,58/61 SSU/12
LSUA 5201-5026 4073-3906 none complete 175/176 LSU/1
LSUB 4618*-4586 3667-3691 mtR_143/27 30/29 LSU/3
LSUC 225*-204 1824-1839 mtR_203/2 23/22 LSU/4
LSUD 5854-5772 3114-3196 mtR_5773a/31 85/83 LSU/8
LSUE 5771-5577 3199-3377 none complete 190/195 LSU/9
LSUF 1516-1630 3507-3395 none complete 125,110/115 LSU/11
LSUG 389-283 5667-5775 mtR_36a/221 110/107 LSU/12
RNA1 593-506 3827-3905 mtR_19/19 95/88 LSU/6
RNA2 1698-1763 3098-3032 mtR_69c/15 75/67 LSU/2
RNA3 1830-1910 5658-5584 mtR_5713a/91 85/81 LSU/7
RNA4 4625-4696   mtR_37a/410 75/72  
RNA5 4716-4802   mtR_5822/61 92/87 SSU/9
RNA6 4808-4865 1643-1584 mtR_92/41 58/58 LSU/15
RNA7 5283-5202 1678-1758 mtR_5837/69 94/82  
RNA8 5954-5855 3031-2940 mtR_41/38 115/100 SSU/5
RNA9 72-125 4211-4183 mtR_182b/7 62/54 SSU/8
RNA10 724-625 4359-4301 mtR_140a/29 100/100 LSU/13
RNA11 5340-5284 2300-2240 mtR_14a/12 78/57 LSU/5
RNA12 4887-4945 mtR_52b/35 68/59 SSU/2  
RNA13 5025-4996 4401-4373 mtR_5844/63 48/30 LSU/10
RNA14 5546-5508 2039-1997 mtR_11c/62 40/39 SSU/1
RNA15 624-594 1759-1790 mtR_76b/163 40/31  
RNA16 3-33   mtR_60/21 ?/31  
RNA17 126-165 2192-2154 mtR_18a/132 ?/40 SSU/3
RNA18 4964-4988 1518-1494 mtR_104/23 ?/25 LSU/14
RNA19 5576-5547   mtR_42/9 ?/30 SSU/7
RNA20 34-71   mtR_86/15 54/38  
RNA21 1807*-1829   mtR_26/26 ?/23  
RNA22 5378-5341   mtR_59/124 ?/38  
RNA23t 171-203   mtR_21/182    
RNA24t 262-224   mtR_39/36    
RNA25t 283-262   mtR_40b/15    
RNA26t 1764-1806   mtR_49/72    
RNA27t 4138-4085   mtR_84/122    

a Naming convention reflects history of identification4. RNA23t through RNA27t are tentative assignments. We have not detected them in RNA blotting experiments, but sequence conservation and comparative abundance of cDNAs29 suggest they are mature RNAs or abundant processing products.

b 5' ends are based on primer extension data, except for RNA1 (RNase protection), RNAs 9 and 14-22 (5' RACE), and RNA21 (Raabe, et al.29 cDNAs and sequence conservation). 3' ends were determined by 3' RACE except for SSUE, LSUB, and LSUC (inferred from 5' end location and transcript size). We have confirming RNase protection data for many of the 5' and 3' ends. *, coordinates inferred.

c cDNAs corresponding to mapped P. falciparum mt RNAs. Each designation is name/number of cDNAs.

d The observed size from denaturing polyacrylamide gels is noted for the RNA size, compared to the gene size (DNA) based on the location of 5' and 3' ends. ?, RNA identified by RACE, not blotting.

e Assignment of P. falciparum small mt RNAs to large and small subunit rRNAs; numbers indicate linear order relative to conventional rRNAs.


References:

  1. Feagin JE, Mericle BL, Werner E, and Morris M (1997). Identification of additional rRNA fragments encoded by the Plasmodium falciparum 6 kb element. Nucleic Acids Res. 25: 438-446.
  2. Raabe CA, Sanchez CP, Randau G, Robeck T, Skryabin BV, et al. (2010). A global view of the nonprotein-coding transcriptome in Plasmodium falciparum. Nucleic Acids Res. 38: 608-617.