Last modified on 09 October 2002.
Literature Reference:
Jackson S.A., Cannone J.J., Lee J.C., Gutell R.R., and Woodson S.A. (2002)
Distribution of rRNA Introns in the Three-Dimensional Structure of the Ribosome.
Running Title: rRNA Introns in 3D.
Journal of Molecular Biology, 323:35-52.
Manuscript Figures and Tables:
Figure/Table Legend | Panel | ||
---|---|---|---|
A | B | Composite | |
Table 1. Number of known intron sequences at each intron position. | 16S rRNA ( HTML ) | 23S rRNA ( HTML ) | |
Table 2. | Conservation of rRNA residues prior to intron insertion sites. ( HTML ) | Number of intron-containing positions adjacent to highly conserved rRNA residues. ( HTML ) | |
Figure 1. Distribution of introns in rRNAs. | 16S rRNA ( JPG ) | 23S rRNA ( JPG ) | |
Figure 2. Location of introns in 16S rRNAs. | Secondary structure ( PS | PDF ) | Three-dimensional structure of 30S ribosome ( PDF | TIFF ) | |
Figure 3. Location of introns in 23S rRNAs. | Secondary structure ( PS | PDF ) | Three-dimensional structure of 50S ribosome ( PDF | TIFF ) | |
Figure 4. Three-dimensional clustering of rRNA intron sites within 70 S ribosome. | Comparison of rRNA positions ( JPG ) | Comparison of intron types ( JPG ) | |
Figure 5. Conservation of rRNAs. | 30S (front view) ( TIFF ) | 50S (crown view) ( TIFF ) | Composite ( PDF | TIFF ) |
Figure 6. Proximity of intron sites to tRNA bound in the 70S ribosome. | Figure 6 ( JPG ) | ||
Figure 7. Surface accessibility of intron positions in mature ribosomal subunits. | 16S Introns ( JPG ) | 23S Introns ( JPG ) | |
Figure 8. Locations of reverse splicing targets in E. coli 23S rRNA. | Crown view ( TIFF ) | Back view ( TIFF ) | Composite ( PDF | TIFF ) |
Additional Figures and Tables:
Secondary Structure Diagrams and RasMol Scripts Showing Intron Positions (from 14 February 2002 data):
Contents | Exon | ||||||
---|---|---|---|---|---|---|---|
16S rRNA | 30S ribosome (1FJF) | 23S rRNA (5' half) | 23S rRNA (3' half) | 50S ribosome (1JJ2) | 16S + 23S + tRNA/70S + tRNA (1GIX + 1GIY) | ||
1) Intron Positions on the Escherichia coli IISTR: Dimensions: A, B, C |
PS | PDF | 16S | 30S | PS | PDF | PS | PDF | 23S | 50S | 16S + 23S + tRNAs | 70S + tRNAs | |
2A) Intron Positions on the Three Phylogenetic Domains Conservation Diagram Dimensions: A, B, C, D |
PS | PDF | 16S | PS | PDF | PS | PDF | 23S | ||
2B) Intron Positions on the Three Phylogenetic Domains/Two Organelles Conservation Diagram (Starting Point for Figures 2A and 3A) Dimensions: A, B, C, D |
PS | PDF | 16S | PS | PDF | PS | PDF | 23S | ||
3) Intron Positions and Color-Coded rRNA Domains Dimensions: B, E |
PS | PDF | 16S | 30S | PS | PDF | PS | PDF | 23S | 50S | ||
Help with RasMol: | Our list of simple RasMol commands. | For further information about RasMol, visit these two sites: |
Conservation and Intron Distribution Information:
16S rRNA | 23S rRNA | |
---|---|---|
Conservation Data | 3P | 3P2O (Text) | 3P | 3P2O (Text) |
Source Data for Table 1: | Table 1A: Manuscript | Current | Table 1B: Manuscript | Current |
Source Data for Tables 2a and 2b: | Manuscript: Full | Summary Current: Full | Summary |
|
Online Table. | Spatial clustering of intron insertion sites within the ribosome (Å). ( HTML ) | |
rRNA Introns Data (CRW Site) | Tables of intron data sorted by various criteria. ( HTML ) |