Conservation categories are defined: Categories A-E: a nucleotide exists in > 95% of the sequences studied. "Category A": the existing nucleotide is 98%-100% conserved. "Category B": the existing nucleotide is 90%-98% conserved. "Category C": the existing nucleotide is 80%-90% conserved. "Category D": the existing nucleotide is < 80% conserved. "Category E": sum of Categories A-D. "Category N": nucleotide exists in < 95% of the sequences studied. Other abbreviations used: IP: intron position. nt: nucleotide.
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL
General/Overall rRNA Nucleotide Conservation Category A 355 (23.0%) 178 (11.5%) 595 (20.5%) 150 (5.2%)
Category B 156 (10.1%) 175 (11.3%) 349 (12.0%) 203 (7.0%)
Category C 80 (5.2%) 116 (7.5%) 283 (9.7%) 168 (5.8%)
Category D 546 (35.4%) 395 (25.6%) 1204 (41.5%) 647 (22.3%)
Category E 1137 (73.7%) 864 (56.0%) 2431 (83.7%) 1168 (40.2%)
Category N 405 (26.3%) 678 (44.0%) 473 (16.3%) 1736 (59.8%)
TOTAL 1542 (100.0%) 1542 (100.0%) 2904 (100.0%) 2904 (100.0%)
(rRNA) Distance to Nearest "Category A" nt 1 Position 530 (34.4%) 274 (17.8%) 913 (31.4%) 240 (8.3%)
2 Positions 117 (7.6%) 77 (5.0%) 219 (7.5%) 68 (2.3%)
3 Positions 89 (5.8%) 66 (4.3%) 161 (5.5%) 51 (1.8%)
4 or More Positions 806 (52.3%) 1125 (73.0%) 1611 (55.5%) 2545 (87.6%)
(rRNA) Distance to Nearest "Category A" or "B" nt 1 Position 713 (46.2%) 505 (32.7%) 1346 (46.3%) 532 (18.3%)
2 Positions 117 (7.6%) 99 (6.4%) 235 (8.1%) 110 (3.8%)
3 Positions 77 (5.0%) 72 (4.7%) 168 (5.8%) 79 (2.7%)
4 or More Positions 635 (41.2%) 866 (56.2%) 1155 (39.8%) 2183 (75.2%)
Intron Positions (IP) All Introns 94 (100.0%) 94 (100.0%) 84 (100.0%) 84 (100.0%)
Group I Introns 46 (100.0%) 46 (100.0%) 44 (100.0%) 44 (100.0%)
Group II Introns 4 (100.0%) 4 (100.0%) 10 (100.0%) 10 (100.0%)
Archaeal Introns 14 (100.0%) 14 (100.0%) 6 (100.0%) 6 (100.0%)
Spliceosomal Introns 27 (100.0%) 27 (100.0%) 26 (100.0%) 26 (100.0%)
Other Introns 12 (100.0%) 12 (100.0%) 3 (100.0%) 3 (100.0%)
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL
All Introns # of Introns (All Introns) Total 94 (100.0%) 94 (100.0%) 84 (100.0%) 84 (100.0%)
Conservation of nt Prior to IP Category A 34 (36.2%) 18 (19.1%) 38 (45.2%) 20 (23.8%)
Category B 17 (18.1%) 16 (17.0%) 10 (11.9%) 16 (19.0%)
Category C 6 (6.4%) 15 (16.0%) 2 (2.4%) 6 (7.1%)
Category D 34 (36.2%) 28 (29.8%) 33 (39.3%) 32 (38.1%)
Category N 3 (3.2%) 17 (18.1%) 1 (1.2%) 10 (11.9%)
Conservation of nt Following IP Category A 43 (45.7%) 20 (21.3%) 33 (39.3%) 19 (22.6%)
Category B 12 (12.8%) 17 (18.1%) 15 (17.9%) 10 (11.9%)
Category C 7 (7.4%) 14 (14.9%) 7 (8.3%) 5 (6.0%)
Category D 29 (30.9%) 25 (26.6%) 28 (33.3%) 38 (45.2%)
Category N 3 (3.2%) 18 (19.1%) 1 (1.2%) 12 (14.3%)
Distance to Nearest "Category A" nt 1 Position 56 (59.6%) 32 (34.0%) 50 (59.5%) 28 (33.3%)
2 Positions 19 (20.2%) 13 (13.8%) 18 (21.4%) 14 (16.7%)
3 Positions 7 (7.4%) 11 (11.7%) 6 (7.1%) 9 (10.7%)
4 or More Positions 12 (12.8%) 38 (40.4%) 10 (11.9%) 33 (39.3%)
IP Adjacent to at least one "Category A" nt 56 (59.6%) 32 (34.0%) 50 (59.5%) 28 (33.3%)
IP within Two Positions of at least one "Category A" nt 75 (79.8%) 45 (47.9%) 68 (81.0%) 42 (50.0%)
IP within Three Positions of at least one "Category A" nt 82 (87.2%) 56 (59.6%) 74 (88.1%) 51 (60.7%)
IP Flanked by Two "Category A" nt 21 (22.3%) 6 (6.4%) 21 (25.0%) 11 (13.1%)
Distance to Nearest "Category B" nt 1 Position 70 (74.5%) 49 (52.1%) 64 (76.2%) 46 (54.8%)
2 Positions 14 (14.9%) 12 (12.8%) 11 (13.1%) 16 (19.0%)
3 Positions 3 (3.2%) 8 (8.5%) 4 (4.8%) 5 (6.0%)
4 or More Positions 7 (7.4%) 25 (26.6%) 5 (6.0%) 17 (20.2%)
IP Adjacent to at least one "Category A" or "B" nt 70 (74.5%) 49 (52.1%) 64 (76.2%) 46 (54.8%)
IP within Two Positions of at least one "Category A" or "B" nt 84 (89.4%) 61 (64.9%) 75 (89.3%) 62 (73.8%)
IP within Three Positions of at least one "Category A" or "B" nt 87 (92.6%) 69 (73.4%) 79 (94.0%) 67 (79.8%)
IP Flanked on Both Sides by "Category E" nt 90 (95.7%) 75 (79.8%) 82 (97.6%) 71 (84.5%)
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL
Group I Introns # of Introns (Group I Introns) Total 46 (100.0%) 46 (100.0%) 44 (100.0%) 44 (100.0%)
Conservation of nt Prior to IP Category A 19 (41.3%) 12 (26.1%) 19 (43.2%) 11 (25.0%)
Category B 3 (6.5%) 5 (10.9%) 5 (11.4%) 4 (9.1%)
Category C 2 (4.3%) 5 (10.9%) --0 (---%) 4 (9.1%)
Category D 19 (41.3%) 15 (32.6%) 20 (45.5%) 22 (50.0%)
Category N 3 (6.5%) 9 (19.6%) --0 (---%) 3 (6.8%)
Conservation of nt Following IP Category A 25 (54.3%) 13 (28.3%) 17 (38.6%) 7 (15.9%)
Category B 3 (6.5%) 8 (17.4%) 8 (18.2%) 7 (15.9%)
Category C 5 (10.9%) 6 (13.0%) 3 (6.8%) 1 (2.3%)
Category D 10 (21.7%) 9 (19.6%) 15 (34.1%) 25 (56.8%)
Category N 3 (6.5%) 10 (21.7%) 1 (2.3%) 4 (9.1%)
Distance to Nearest "Category A" nt 1 Position 31 (67.4%) 20 (43.5%) 25 (56.8%) 13 (29.5%)
2 Positions 7 (15.2%) 6 (13.0%) 11 (25.0%) 9 (20.5%)
3 Positions 2 (4.3%) 6 (13.0%) 3 (6.8%) 4 (9.1%)
4 or More Positions 6 (13.0%) 14 (30.4%) 5 (11.4%) 18 (40.9%)
IP Adjacent to at least one "Category A" nt 31 (67.4%) 20 (43.5%) 25 (56.8%) 13 (29.5%)
IP within Two Positions of at least one "Category A" nt 38 (82.6%) 26 (56.5%) 36 (81.8%) 22 (50.0%)
IP within Three Positions of at least one "Category A" nt 40 (87.0%) 32 (69.6%) 39 (88.6%) 26 (59.1%)
IP Flanked by Two "Category A" nt 13 (28.3%) 5 (10.9%) 11 (25.0%) 5 (11.4%)
Distance to Nearest "Category B" nt 1 Position 33 (71.7%) 26 (56.5%) 33 (75.0%) 22 (50.0%)
2 Positions 6 (13.0%) 5 (10.9%) 7 (15.9%) 10 (22.7%)
3 Positions 2 (4.3%) 3 (6.5%) 2 (4.5%) 3 (6.8%)
4 or More Positions 5 (10.9%) 12 (26.1%) 2 (4.5%) 9 (20.5%)
IP Adjacent to at least one "Category A" or "B" nt 33 (71.7%) 26 (56.5%) 33 (75.0%) 22 (50.0%)
IP within Two Positions of at least one "Category A" or "B" nt 39 (84.8%) 31 (67.4%) 40 (90.9%) 32 (72.7%)
IP within Three Positions of at least one "Category A" or "B" nt 41 (89.1%) 34 (73.9%) 42 (95.5%) 35 (79.5%)
IP Flanked on Both Sides by "Category E" nt 42 (91.3%) 36 (78.3%) 43 (97.7%) 40 (90.9%)
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL
Group II Introns # of Introns (Group II Introns) Total 4 (100.0%) 4 (100.0%) 10 (100.0%) 10 (100.0%)
Conservation of nt Prior to IP Category A 2 (50.0%) 1 (25.0%) 6 (60.0%) 5 (50.0%)
Category B --0 (---%) 1 (25.0%) 2 (20.0%) 2 (20.0%)
Category C 1 (25.0%) --0 (---%) --0 (---%) --0 (---%)
Category D 1 (25.0%) 2 (50.0%) 2 (20.0%) 3 (30.0%)
Category N --0 (---%) --0 (---%) --0 (---%) --0 (---%)
Conservation of nt Following IP Category A --0 (---%) --0 (---%) 4 (40.0%) 4 (40.0%)
Category B 1 (25.0%) --0 (---%) 3 (30.0%) --0 (---%)
Category C 2 (50.0%) 3 (75.0%) --0 (---%) 1 (10.0%)
Category D 1 (25.0%) 1 (25.0%) 3 (30.0%) 5 (50.0%)
Category N --0 (---%) --0 (---%) --0 (---%) --0 (---%)
Distance to Nearest "Category A" nt 1 Position 2 (50.0%) 1 (25.0%) 6 (60.0%) 6 (60.0%)
2 Positions 2 (50.0%) 2 (50.0%) 3 (30.0%) 2 (20.0%)
3 Positions --0 (---%) 1 (25.0%) 1 (10.0%) 1 (10.0%)
4 or More Positions --0 (---%) --0 (---%) --0 (---%) 1 (10.0%)
IP Adjacent to at least one "Category A" nt 2 (50.0%) 1 (25.0%) 6 (60.0%) 6 (60.0%)
IP within Two Positions of at least one "Category A" nt 4 (100.0%) 3 (75.0%) 9 (90.0%) 8 (80.0%)
IP within Three Positions of at least one "Category A" nt 4 (100.0%) 4 (100.0%) 10 (100.0%) 9 (90.0%)
IP Flanked by Two "Category A" nt --0 (---%) --0 (---%) 4 (40.0%) 3 (30.0%)
Distance to Nearest "Category B" nt 1 Position 3 (75.0%) 2 (50.0%) 9 (90.0%) 7 (70.0%)
2 Positions 1 (25.0%) 1 (25.0%) 1 (10.0%) 3 (30.0%)
3 Positions --0 (---%) 1 (25.0%) --0 (---%) --0 (---%)
4 or More Positions --0 (---%) --0 (---%) --0 (---%) --0 (---%)
IP Adjacent to at least one "Category A" or "B" nt 3 (75.0%) 2 (50.0%) 9 (90.0%) 7 (70.0%)
IP within Two Positions of at least one "Category A" or "B" nt 4 (100.0%) 3 (75.0%) 10 (100.0%) 10 (100.0%)
IP within Three Positions of at least one "Category A" or "B" nt 4 (100.0%) 4 (100.0%) 10 (100.0%) 10 (100.0%)
IP Flanked on Both Sides by "Category E" nt 4 (100.0%) 4 (100.0%) 10 (100.0%) 10 (100.0%)
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL
Archaeal Introns # of Introns (Archaeal Introns) Total 14 (100.0%) 14 (100.0%) 6 (100.0%) 6 (100.0%)
Conservation of nt Prior to IP Category A 8 (57.1%) 4 (28.6%) 5 (83.3%) 3 (50.0%)
Category B 5 (35.7%) 7 (50.0%) --0 (---%) 2 (33.3%)
Category C --0 (---%) 1 (7.1%) 1 (16.7%) --0 (---%)
Category D 1 (7.1%) 1 (7.1%) --0 (---%) --0 (---%)
Category N --0 (---%) 1 (7.1%) --0 (---%) 1 (16.7%)
Conservation of nt Following IP Category A 8 (57.1%) 4 (28.6%) 4 (66.7%) 4 (66.7%)
Category B 2 (14.3%) 4 (28.6%) --0 (---%) --0 (---%)
Category C --0 (---%) 1 (7.1%) 1 (16.7%) --0 (---%)
Category D 4 (28.6%) 4 (28.6%) 1 (16.7%) --0 (---%)
Category N --0 (---%) 1 (7.1%) --0 (---%) 2 (33.3%)
Distance to Nearest "Category A" nt 1 Position 11 (78.6%) 7 (50.0%) 5 (83.3%) 4 (66.7%)
2 Positions 1 (7.1%) 1 (7.1%) --0 (---%) --0 (---%)
3 Positions 1 (7.1%) --0 (---%) --0 (---%) --0 (---%)
4 or More Positions 1 (7.1%) 6 (42.9%) 1 (16.7%) 2 (33.3%)
IP Adjacent to at least one "Category A" nt 11 (78.6%) 7 (50.0%) 5 (83.3%) 4 (66.7%)
IP within Two Positions of at least one "Category A" nt 12 (85.7%) 8 (57.1%) 5 (83.3%) 4 (66.7%)
IP within Three Positions of at least one "Category A" nt 13 (92.9%) 8 (57.1%) 5 (83.3%) 4 (66.7%)
IP Flanked by Two "Category A" nt 5 (35.7%) 1 (7.1%) 4 (66.7%) 3 (50.0%)
Distance to Nearest "Category B" nt 1 Position 13 (92.9%) 12 (85.7%) 5 (83.3%) 5 (83.3%)
2 Positions 1 (7.1%) --0 (---%) --0 (---%) --0 (---%)
3 Positions --0 (---%) 1 (7.1%) --0 (---%) --0 (---%)
4 or More Positions --0 (---%) 1 (7.1%) 1 (16.7%) 1 (16.7%)
IP Adjacent to at least one "Category A" or "B" nt 13 (92.9%) 12 (85.7%) 5 (83.3%) 5 (83.3%)
IP within Two Positions of at least one "Category A" or "B" nt 14 (100.0%) 12 (85.7%) 5 (83.3%) 5 (83.3%)
IP within Three Positions of at least one "Category A" or "B" nt 14 (100.0%) 13 (92.9%) 5 (83.3%) 5 (83.3%)
IP Flanked on Both Sides by "Category E" nt 14 (100.0%) 13 (92.9%) 6 (100.0%) 4 (66.7%)
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL
Spliceosomal Introns # of Introns (Spliceosomal Introns) Total 27 (100.0%) 27 (100.0%) 26 (100.0%) 26 (100.0%)
Conservation of nt Prior to IP Category A 4 (14.8%) --0 (---%) 10 (38.5%) 3 (11.5%)
Category B 9 (33.3%) 3 (11.1%) 3 (11.5%) 8 (30.8%)
Category C 3 (11.1%) 9 (33.3%) 1 (3.8%) 2 (7.7%)
Category D 11 (40.7%) 8 (29.6%) 11 (42.3%) 7 (26.9%)
Category N --0 (---%) 7 (25.9%) 1 (3.8%) 6 (23.1%)
Conservation of nt Following IP Category A 10 (37.0%) 3 (11.1%) 9 (34.6%) 5 (19.2%)
Category B 4 (14.8%) 3 (11.1%) 5 (19.2%) 3 (11.5%)
Category C --0 (---%) 3 (11.1%) 2 (7.7%) 3 (11.5%)
Category D 13 (48.1%) 10 (37.0%) 10 (38.5%) 9 (34.6%)
Category N --0 (---%) 8 (29.6%) --0 (---%) 6 (23.1%)
Distance to Nearest "Category A" nt 1 Position 12 (44.4%) 3 (11.1%) 16 (61.5%) 7 (26.9%)
2 Positions 8 (29.6%) 4 (14.8%) 5 (19.2%) 3 (11.5%)
3 Positions 3 (11.1%) 3 (11.1%) 2 (7.7%) 4 (15.4%)
4 or More Positions 4 (14.8%) 17 (63.0%) 3 (11.5%) 12 (46.2%)
IP Adjacent to at least one "Category A" nt 12 (44.4%) 3 (11.1%) 16 (61.5%) 7 (26.9%)
IP within Two Positions of at least one "Category A" nt 20 (74.1%) 7 (25.9%) 21 (80.8%) 10 (38.5%)
IP within Three Positions of at least one "Category A" nt 23 (85.2%) 10 (37.0%) 23 (88.5%) 14 (53.8%)
IP Flanked by Two "Category A" nt 2 (7.4%) --0 (---%) 3 (11.5%) 1 (3.8%)
Distance to Nearest "Category B" nt 1 Position 19 (70.4%) 7 (25.9%) 20 (76.9%) 14 (53.8%)
2 Positions 6 (22.2%) 6 (22.2%) 3 (11.5%) 4 (15.4%)
3 Positions 1 (3.7%) 2 (7.4%) 2 (7.7%) 2 (7.7%)
4 or More Positions 1 (3.7%) 12 (44.4%) 1 (3.8%) 6 (23.1%)
IP Adjacent to at least one "Category A" or "B" nt 19 (70.4%) 7 (25.9%) 20 (76.9%) 14 (53.8%)
IP within Two Positions of at least one "Category A" or "B" nt 25 (92.6%) 13 (48.1%) 23 (88.5%) 18 (69.2%)
IP within Three Positions of at least one "Category A" or "B" nt 26 (96.3%) 15 (55.6%) 25 (96.2%) 20 (76.9%)
IP Flanked on Both Sides by "Category E" nt 27 (100.0%) 18 (66.7%) 25 (96.2%) 19 (73.1%)
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL
Other Introns # of Introns (Other Introns) Total 12 (100.0%) 12 (100.0%) 3 (100.0%) 3 (100.0%)
Conservation of nt Prior to IP Category A 5 (41.7%) 5 (41.7%) 1 (33.3%) --0 (---%)
Category B 1 (8.3%) --0 (---%) --0 (---%) 1 (33.3%)
Category C --0 (---%) 1 (8.3%) --0 (---%) --0 (---%)
Category D 6 (50.0%) 6 (50.0%) 2 (66.7%) 2 (66.7%)
Category N --0 (---%) --0 (---%) --0 (---%) --0 (---%)
Conservation of nt Following IP Category A 5 (41.7%) 2 (16.7%) --0 (---%) --0 (---%)
Category B 2 (16.7%) 3 (25.0%) 1 (33.3%) --0 (---%)
Category C 2 (16.7%) 3 (25.0%) 1 (33.3%) 1 (33.3%)
Category D 3 (25.0%) 3 (25.0%) 1 (33.3%) 2 (66.7%)
Category N --0 (---%) 1 (8.3%) --0 (---%) --0 (---%)
Distance to Nearest "Category A" nt 1 Position 8 (66.7%) 6 (50.0%) 1 (33.3%) --0 (---%)
2 Positions 2 (16.7%) 1 (8.3%) 1 (33.3%) --0 (---%)
3 Positions 1 (8.3%) 1 (8.3%) --0 (---%) 1 (33.3%)
4 or More Positions 1 (8.3%) 4 (33.3%) 1 (33.3%) 2 (66.7%)
IP Adjacent to at least one "Category A" nt 8 (66.7%) 6 (50.0%) 1 (33.3%) --0 (---%)
IP within Two Positions of at least one "Category A" nt 10 (83.3%) 7 (58.3%) 2 (66.7%) --0 (---%)
IP within Three Positions of at least one "Category A" nt 11 (91.7%) 8 (66.7%) 2 (66.7%) 1 (33.3%)
IP Flanked by Two "Category A" nt 2 (16.7%) 1 (8.3%) --0 (---%) --0 (---%)
Distance to Nearest "Category B" nt 1 Position 10 (83.3%) 8 (66.7%) 1 (33.3%) 1 (33.3%)
2 Positions 1 (8.3%) 1 (8.3%) 1 (33.3%) 1 (33.3%)
3 Positions --0 (---%) 1 (8.3%) --0 (---%) --0 (---%)
4 or More Positions 1 (8.3%) 2 (16.7%) 1 (33.3%) 1 (33.3%)
IP Adjacent to at least one "Category A" or "B" nt 10 (83.3%) 8 (66.7%) 1 (33.3%) 1 (33.3%)
IP within Two Positions of at least one "Category A" or "B" nt 11 (91.7%) 9 (75.0%) 2 (66.7%) 2 (66.7%)
IP within Three Positions of at least one "Category A" or "B" nt 11 (91.7%) 10 (83.3%) 2 (66.7%) 2 (66.7%)
IP Flanked on Both Sides by "Category E" nt 12 (100.0%) 11 (91.7%) 3 (100.0%) 3 (100.0%)
Information 16S rRNA 23S rRNA
3DOM ALL 3DOM ALL