SASA values were calculated using the GETAREA program (reference: "Exact and Efficient Analytical Calculation of the Accessible Surface Areas and their Gradients for Macromolecules," J. Comp. Chem. 19:319-333 (1998).
Protein: ribosomal protein interacting with the LPTL in the ribosomal crystal structures.
E. colia Xtal b RNA c RNAP d
16S rRNA
64 64 G 196.77 196.77
65 65 U 117.60 117.60
66 66 G 37.44 37.44
67 67 C 47.60 47.60
68 68 G 96.22 96.22
Average 99.13 99.13
Protein none
 
323 318 U 153.77 42.88
324 319 G 158.80 84.56
325 320 A 133.80 74.46
326 321 G 100.15 91.83
327 322 A 75.34 75.34
Average 124.37 73.81
Protein S20
 
934 911 C 107.37 107.37
935 912 A 90.24 74.36
936 913 C 88.18 83.57
937 914 A 125.05 123.39
938 915 A 110.13 86.56
Average 104.19 95.05
Protein none
 
956 933 U 166.32 130.38
957 934 U 145.60 104.78
958 935 A 194.47 93.93
959 936 A 70.04 53.26
960 937 U 81.06 68.16
Average 131.50 90.10
Protein S19
 
1053 1035 G 72.80 72.80
1054 1036 C 185.43 181.04
1055 1037 A 117.98 37.90
1056 1038 U 122.38 35.19
1057 1039 G 157.20 53.39
Average 131.16 76.06
Protein S3
 
1177 1158 G 167.30 158.16
1178 1159 G 166.21 118.29
1179 1160 A 134.00 48.34
1180 1161 A 98.13 65.20
1181 1162 G 82.54 82.54
Average 129.64 94.51
Protein S10
 
1315 1296 U 90.88 76.26
1316 1297 G 132.38 85.34
1317 1298 C 252.13 122.89
1318 1299 A 133.23 43.33
1319 1300 A 92.69 21.83
Average 140.26 69.93
Protein S14, S19
 
 
 
 
 
 
 
 
 
E. colia Xtal b RNA c RNAP d
23S rRNA
131 125 U 251.67 251.67
-- 126 C -- --
-- 127 U -- --
-- 128 A 238.24 238.24
148 129 A 167.96 167.96
Average -- --
Protein none
 
306 313 U 112.29 111.08
307 314 G 146.07 146.07
308 315 G 113.66 101.77
309 316 A 77.30 49.23
310 317 A 102.59 53.02
Average 110.38 92.23
Protein L24
 
319 326 G 97.50 71.23
320 327 A 189.31 45.81
321 328 U 238.79 103.61
322 329 A 84.02 29.50
323 330 C 150.53 36.63
Average 152.03 57.36
Protein L4
 
328 335 U 162.20 107.52
329 336 G 135.08 37.60
330 337 A 53.57 53.57
331 338 C 124.91 92.68
332 339 A 110.42 61.15
Average 117.24 70.50
Protein L4, L24
 
475 481 U 70.93 70.93
476 482 G 83.09 83.09
477 483 C 72.94 60.82
478 484 A 145.40 63.78
479 485 A 103.31 77.20
Average 95.13 71.16
Protein L24
 
476 482 G 83.09 83.09
477 483 C 72.94 60.82
478 484 A 145.40 63.78
479 485 A 103.31 77.20
480 486 A 149.67 34.72
Average 110.88 63.92
Protein L24
 
499 505 C 116.94 94.39
500 506 G 109.88 107.50
501 507 A 123.90 120.69
502 508 A 112.86 112.86
503 509 A 81.54 81.54
Average 109.02 103.40
Protein none
 
 
567 624 U 111.51 56.90
568 625 U 109.53 67.02
569 626 U 61.16 61.16
570 627 G 34.69 34.69
571 628 A 69.82 35.72
Average 73.34 51.10
Protein L15
E. colia Xtal b RNA c RNAP d
23S rRNA (continued)
1082 1186 C 165.80 165.80
1083 1187 U 181.50 181.50
1084 1188 A 220.02 183.38
1085 1189 A 79.99 79.99
1086 1190 G 68.50 68.50
Average 143.16 135.83
Protein none
 
1282 1388 U 155.25 129.19
1283 1389 G 161.79 161.79
1284 1390 A 177.56 177.56
1285 1391 G 137.73 137.73
1286 1392 A 110.72 110.72
Average 148.61 143.40
Protein none
 
1565 1651 C 124.40 47.17
-- 1652 C 312.76 43.52
1566 1653 A 254.45 119.94
1567 1654 U 197.09 42.41
1568 1655 G 180.66 132.70
Average 213.87 77.15
Protein L2, L37ae
 
1752 1808 C 109.57 109.57
1753 1809 G 133.12 132.39
1754 1810 C 182.14 86.52
1755 1811 A 120.16 120.16
1756 1812 G 61.44 61.29
Average 121.29 101.99
Protein L24e
 
1925 1966 U 152.51 152.51
1926 1967 U 204.43 204.43
1927 1968 A 91.31 91.31
1928 1969 A 78.20 78.20
1929 1970 G 119.80 119.80
Average 129.25 129.25
Protein none
 
2447 2482 G 49.99 49.99
2448 2483 A 81.31 76.08
2449 2484 U 83.58 83.58
2450 2485 A 21.34 21.34
2451 2486 A 140.34 140.34
Average 75.31 75.31
Protein none
 
2562 2597 U 167.13 87.96
2563 2598 U 146.85 77.07
2564 2599 A 79.28 65.66
2565 2600 A 65.95 55.83
2566 2601 A 115.95 93.25
Average 115.03 75.95
Protein L14
 
5S rRNA
24 22 G 92.14 92.14
25 23 U 289.47 289.47
-- 24 U 210.16 202.85
26 25 G 101.99 94.09
27 26 C 89.57 41.03
Average 156.67 143.92
Protein none

  1. Position numbers based on the E. coli 16S and 23S rRNA sequences (GenBank accession #J01695).
  2. The crystal structures of the T. thermophilus 30 S (PDB entry 1FJF; Wimberly et al. 2000) and H. marismortui 50 S (PDB entry 1JJ2; Ban et al. 2000) ribosomal subunits were used for the SASA calculation.
  3. SASAs obtained in the presence of RNA only.
  4. SASAs obtained in the presence of RNA and proteins.