| Haloferax volcanii | Methanosprillum hungatei | |||
|---|---|---|---|---|
| Optimal Accuracy1 | 80% | 46% | ||
| Total Fold Predictions (Optimal + Suboptimal) | 750 | 750 | ||
| Total Pairwise Comparisions | 280,875 | 280,875 | ||
| Structural variation score of 1 to 50 | ||||
| Num of Pairwise Comparisions | 32,378 | 12%2 | 11,016 | 4% |
| ΔΔG Min (kcal/mol) | 0 | 0 | ||
| ΔΔG Max (kcal/mol) | 11 | 8.6 | ||
| ΔΔG Average (kcal/mol) | 2.84 | 2.19 | ||
| Structural variation score of 51 to 100 | ||||
| Num of Pairwise Comparisions | 102,071 | 36% | 32,360 | 12% |
| ΔΔG Min (kcal/mol) | 0 | 0 | ||
| ΔΔG Max (kcal/mol) | 11 | 8.6 | ||
| ΔΔG Average (kcal/mol) | 2.53 | 2.19 | ||
| Structural variation score of 101 to 500 | ||||
| Num of Pairwise Comparisions | 121,805 | 43% | 134,037 | 48% |
| ΔΔG Min (kcal/mol) | 0 | 0 | ||
| ΔΔG Max (kcal/mol) | 11 | 8.6 | ||
| ΔΔG Average (kcal/mol) | 2.24 | 1.74 | ||
| Structural variation score of 501+ | ||||
| Num of Pairwise Comparisions | 24,621 | 9% | 103,462 | 37% |
| ΔΔG Min (kcal/mol) | 0 | 0 | ||
| ΔΔG Max (kcal/mol) | 11 | 8.6 | ||
| ΔΔG Average (kcal/mol) | 2.24 | 1.82 | ||
Both sequences are 16S rRNAs. For each sequence, MFold 3.1 predicts one optimal or minimum free energy fold and 749 suboptimals folds (750 total folds). Pairwise comparisions are grouped based on the structural variation between the two folds compared. For details on how structural variation between two folds is calculated see Suboptimal Structural Variation Score in Methods. The range of ΔΔG values observed is 0-11 kcal/mol for H. volcanii and 0-8.60 kcal/mol for M. hungatei, and all ΔG values are pre-efn2 re-evaluation.
1 Without efn2 re-evaluation and re-ordering of predicted folds.
2 Percentage of total pairwise comparisions.
