Table 1. List of GenBank annotations and accession numbers for small subunit (18S) rDNA sequences used in this study. The outline of the valve was coded so this character could be mapped onto phylogenetic trees: 0=circular/subcircular, 2=elongate/biangular, 3=triangular, 4=quadrangular. The outline codings were based on generic descriptions from Round et al. (1990). Unidentified sequences were coded with "?", and outgroup taxa were coded "NA."
Taxon (GenBank Annotation) Outline Coding GenBank Accession ID
Melosira varians Agardh 0 X85402
Melosira varians Agardh 0 AJ243065
Stephanopyxis cf. broschii 0 M87330
Aulacoseira baicalensis (K. Meyer) Simonsen 0 AY121821
Aulacoseira islandica (O. Müller) Simonsen 0 AY121820
Aulacoseira ambigua (Grunow) Simonsen 0 X85404
Aulacoseira nyassensis (O. Müller) Simonsen 0 AJ535187
Aulacoseira nyassensis (O. Müller) Simonsen 0 AY121819
Aulacoseira distans (Ehrenburg) Simonsen 0 X85403
Aulacoseira baicalensis (K. Meyer) Simonsen 0 AJ535186
Aulacoseira baicalensis (K. Meyer) Simonsen 0 AJ535185
Aulacoseira islandica (O. Müller) Simonsen 0 AJ535183
Aulacoseira skvortzowii Edlund, Stoermer, and Taylor 0 AJ535184
Aulacoseira skvortzowii Edlund, Stoermer, and Taylor 0 AY121822
Aulacoseira subarctica (O. Müller) Haworth 0 AY121818
Actinocyclus curvatulus Janisch 0 X85401
Actinoptychus seniarius (Ehrenberg) Héribaud 0 AJ535182
Coscinodiscus radiatus Ehrenberg 0 X77705
Rhizosolenia setigera Brightwell 0 M87329
Guinardia flaccida (Castracane) H. Peragallo 0 AJ535191
Guinardia delicatula (Cleve) Hasle 0 AJ535192
Corethron hystrix Hensen 0 AJ535179
Corethron criophilum Castracane 0 X85400
Corethron inerme Karsten 0 AJ535180
Paralia sol (Ehrenberg) Crawford 0 AJ535174
Proboscia alata Brightwell (Sundström) 0 AJ535181
Leptocylindrus danicus Cleve 0 AJ535175
Leptocylindrus minimus Gran 0 AJ535176
Thalassiosira oceanica Hasle 0 AF374479
Thalassiosira weissflogii (Grunow) Fryxell & Hasle 0 AF374477
Thalassiosira rotula Muenier 0 AF462058
Thalassiosira rotula Muenier 0 AF462059
Thalassiosira rotula Muenier 0 AF374480
Rhizosolenia imbricate Brightwell 0 AJ535178
Rhizosolenia similoides Cleve-Euler 0 AJ535177
Papiliocellulus elegans Hasle, von Stosch et Syvertsen 2 X85388
Ditylum brightwellii (West) Grunow in van Heurck 2/3/4 X85386
Ditylum brightwellii (West) Grunow in van Heurck 2/3/4 AY188181
Ditylum brightwellii (West) Grunow in van Heurck 2/3/4 AY188182
Bellerochea malleus (Brightwell) van Heurck 2/3/4 AF525671
Lithodesmium undulatum Ehrenberg 3/4 Y10569
Helicotheca tamesis (Schrubsole) Ricard 2 X85385
Lauderia borealis Cleve 0 X85399
Porosira pseudodenticulata Hustedt (Jousé) 0 X85398
Detonula confervacea (Cleve) Gran 0 AF525672
Thalassiosira guillardii Hasle 0 AF374478
Thalassiosira weissflogii (Grunow) Fryxell & Hasle 0 AJ535170
Skeletonema menzellii Guillard, Carpenter et Reim 0 AJ536450
Skeletonema menzellii Guillard, Carpenter et Reim 0 AJ535168
Skeletonema pseudocostatum Medlin 0 AF462060
Skeletonema sp. 0 AJ535165
Skeletonema subsalsum (Cleve-Euler) Bethge 0 AJ535166
Skeletonema costatum (Grev.) Cleve 0 X85395
Skeletonema costatum (Grev.) Cleve 0 X52006
Skeletonema pseudocostatum Medlin 0 X85393
Skeletonema pseudocostatum Medlin 0 X85394
Planktoniella sol (Wallich) Schütt 0 AJ535173
Thalassiosira eccentrica (Ehrenb.) Cleve 0 X85396
Thalassiosira pseudonana Hasle & Heimdal 0 AJ535169
Thalassiosira sp. 0 AJ535171
Thalassiosira pseudonana Hasle & Heimdal 0 AF374481
Thalassiosira rotula Muenier 0 X85397
Cyclotella meneghiniana Kützing 0 AJ535172
Cyclotella meneghiniana Kützing 0 AY496206
Cyclotella meneghiniana Kützing 0 AY496207
Cyclotella meneghiniana Kützing 0 AY496210
Cyclotella meneghiniana Kützing 0 AY496211
Cyclotella meneghiniana Kützing 0 AY496212
Cyclotella meneghiniana Kützing 0 AY496213
Cyclotella cf. scaldensis 0 AY496208
Cyclotella cf. scaldensis 0 AY496209
Chaetoceros sp. 2 X85390
Chaetoceros didymus Ehrenb 2 X85392
Chaetoceros debilis Cleve 2 AY229896
Chaetoceros gracilis Schütt 2 AY229897
Chaetoceros curvisetus Cleve 2 AY229895
Biddulphiopsis titiana (Grunow) von Stosch et Simonsen 2 AF525669
Lampriscus kittonii Schmidt 0/3/4 AF525667
Eucampia antarctica (Castracane) Mangin 2 X85389
Chaetoceros rostratus Lauder 2 X85391
Chaetoceros sp. 2 AJ535167
Pleurosira cf. laevis 0 AJ535188
Cymatosira belgica Grunow 2 X85387
Pleurosira laevis (Ehrenberg) Compère 0 AF525670
Odontella sinensis (Greville) Grunow 2 Y10570
Chaetoceros sp. 2 AF145226
Eunotia pectinalis (Dillwyn) Rabenhorst 2 AB085832
Eunotia monodon var. asiatica Skvortzow 2 AB085831
Eunotia formica var. sumatrana Hustedt 2 AB085830
Eunotia sp. 2 AJ535145
Eunotia cf. pectinalis f. minor 2 AJ535146
uncultured diatom ? AY180015
Navicula cryptocephala var. veneta (Kützing) Grunow 2 AJ297724
Pseudogomphonema sp. 2 AF525663
Pleurosigma sp. 2 AF525664
Fragilariopsis sublineata Hasle 2 AF525665
Thalassiosira antarctica Comber1 ? AF374482
Nitzschia apiculata (Gregory) Grunow 2 M87334
Undatella sp. 2 AJ535163
Rossia sp. 2 AJ535144
Amphora cf. capitellata 2 AJ535158
Amphora montana Krasske 2 AJ243061
Planothidium lanceolatum (Brébisson ex Kützing) F. E. Round & L. Bukhtiyarova 2 AJ535189
Lyrella atlantica (Schmidt) D.G. Mann 2 AJ544659
Cymbella cymbiformis C. Agardh 2 AJ535156
Surirella fastuosa var. cuneata (A. Schmidt) H. Peragallo & M. Peragallo 2 AJ535161
Campylodiscus ralfsii Gregory 2 AJ535162
Anomoeoneis sp. haerophora 2 AJ535153
Gomphonema pseudoaugur Lange-Bertalot 2 AB085833
Gomphonema parvulum Kützing 2 AJ243062
Entomoneis cf. alata 2 AJ535160
Lyrella sp. 2 AJ535149
Eolimna subminuscula (Manguin) Gerd Moser 2 AJ243064
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544645
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544651
Sellaphora pupula f. capitata (Skvortsov & K.I. Mey.) Poulin in Poulin, Hamilton & Proulx 2 AJ535155
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544646
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544647
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544648
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544649
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544650
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544652
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544653
Sellaphora pupula (Kützing) Mereschkovsky 2 AJ544654
Sellaphora laevissima (Kützing) D.G. Mann 2 AJ544655
Sellaphora laevissima (Kützing) D.G. Mann 2 AJ544656
Pinnularia cf. interrupta 2 AJ544658
Pinnularia sp. 2 AJ535154
Navicula pelliculosa (Brébisson ex Kützing) Hilse 2 AJ544657
Amphora cf. proteus 2 AJ535147
Eolimna minima (Grunow) Lange-Bertalot 2 AJ243063
Cylindrotheca closterium (Ehrenberg) Reimann et Lewin 2 M87326
Bacillaria paxillifer (O.F. Müller) Hendey 2 M87325
Nitzschia frustulum (Kützing) Grunow 2 AJ535164
Navicula diserta Hustedt 2 AJ535159
Pseudogomphonema sp. 2 AJ535152
Phaeodactylum tricornutum Bohlin 2 AJ269501
Achnanthes bongrainii (M. Peragallo) A. Mann 2 AJ535150
Achnanthes sp. 2 AJ535151
Pseudo-nitzschia multiseries (Hasle) Hasle 2 U18241
Pseudo-nitzschia pungens (Grunow ex Cleve) Hasle 2 U18240
Cocconeis cf. molesta 2 AJ535148
Encyonema triangulatum Kützing 2 AJ535157
uncultured eukaryote ? AY082977
uncultured eukaryote ? AY082992
uncultured diatom ? AY180016
uncultured diatom ? AY180014
Peridinium foliaceum endosymbiont ? Y10567
Peridinium balticum endosymbiont ? Y10566
uncultured diatom ? AY180017
Asterionellopsis glacialis (Castracane) F.E. Round 2 X77701
Talaroneis posidoniae Kooistra & De Stefano 2 AY216905
Asterionellopsis glacialis (Castracane) Round 2 AY216904
Asterionellopsis kariana (Grunow) F.E. Round 2 Y10568
Convoluta convolute diatom endosymbiont ? AY345013
Cyclophora tenuis Castracane 2 AJ535142
Diatoma tenue Agardh 2 AJ535143
Diatoma hyemalis (Roth) Heiberg 2 AB085829
Grammatophora oceanica Ehrenberg 2 AF525655
Grammatophora gibberula Kutzing 2 AF525656
Grammatophora marina (Lyngbe) Kützing 2 AY216906
Licmophora juergensii Agardh 2 AF525661
Rhabdonema sp. 2 AF525660
Asterionella formosa Hassall 2 AF525657
Rhaphoneis belgica (Grunow in van Heurck) Grunow in van Heurck 2 X77703
Staurosira construens Ehrenberg 2 AF525659
Nanofrustulum shiloi (Lee, Reimer & McEmery) Round, Hallsteinsen et Paasche 2 AF525658
Thalassionema sp. 2 AJ535140
Striatella unipunctata (Lyngbye) Agardh 2 AF525666
Hyalosira delicatula Kützing 2 AF525654
Fragilaria sp. 2 AJ535141
Fragilaria cf. islandica 2 AJ535190
Fragilaria striatula Lyngbye 2 X77704
Tabularia tabulata (Agardh) D.M. Williams & Round 2 AY216907
Fragilariforma virescens (Ralfs) D.M. Williams & Round 2 AJ535137
Synedra ulna (Nitzsh) Ehrenberg 2 AJ535139
Fragilaria crotonensis Kitton 2 AF525662
Thalassionema nitzshcioides (Grunow) Hustedt 2 X77702
Synedra sp. 2 AJ535138
Toxarium undulatum Bailey 2 AF525668
uncultured marine diatom ? AF290085
uncultured diatom ? AY180020
Bolidomonas mediterranea Guillou et Chréteinnot-Dinet NA AF123596
Bolidomonas pacifica Guillou et Chréteinnot-Dinet NA AF167153
Bolidomonas pacifica Guillou et Chréteinnot-Dinet NA AF167154
Bolidomonas pacifica Guillou et Chréteinnot-Dinet NA AF167155
Bolidomonas pacifica Guillou et Chréteinnot-Dinet NA AF167156
Bolidomonas pacifica Guillou et Chréteinnot-Dinet NA AF123595
Bolidomonas pacifica Guillou et Chréteinnot-Dinet NA AF167157

1  This sequence is presumably misannotated because it is consistently placed within the pennate diatoms. Small subunit rDNA from cultures confirmed to be T. antarctica is placed with other species of Thalassiosirales (AJA, unpublished data).